Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: In vitro evaluation of osteogenic differentiation on Mg 2+ -releasing piezoelectric scaffolds. A) ALP staining of BMSCs cultured with WH Gel and PWH Gel (Scale bar: 1 mm). B) ARS staining of BMSCs cultured with WH Gel and PWH Gel (Scale bar: 1 mm). C-F) RT-qPCR results showing the relative mRNA expression of OPN, RUNX2, OCN, and COL-I in BMSCs cultured with cryogels for 7 days and 14 days. CLSM images showing the expression of (G) OPN, (H) RUNX2, (I) OCN, and (J) COL-I in BMSCs co-cultured with WH Gel and PWH Gel (Scale bar: 50 μm). Data are presented as mean ± S.D. (n = 3 independent replicates). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; NS, not significant.

Article Snippet: Immunohistochemical staining was carried out for COL-I (Proteintech, 28083-1-AP, USA).

Techniques: In Vitro, Staining, Cell Culture, Quantitative RT-PCR, Expressing

Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: In vivo assessments of large segmental bone defect regeneration using Mg 2+ -releasing piezoelectric scaffold. A-B) Schematic showing the surgical procedure for scaffold implantation in rat radial defects (Scale bar = 1 cm). C) Macroscopic images of the defect site at 6- and 12- weeks post-implantation. D) RUS scores for radial repair. E) 3D micro-CT images of the defects at 6- and 12- weeks post-implantation (Scale bar = 3 mm). F-G) Quantitative micro-CT analysis of BV/TV and trabecular number (Tb.N) in cryogel-treated regions at 6- and 12- weeks post-implantation. H) Representative H&E and Masson's trichrome staining images of defect tissues at 6- and 12-weeks post-implantation (Scale bar: 1 mm). I) Immunohistochemical staining for COL-I (Scale bar: 1 mm). J) Representative immunofluorescence staining of CD31 (Scale bar: 1 mm). Data are expressed as mean ± S.D. (n = 3 independent replicates). Statistical significance was determined as ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; NS, not significant.

Article Snippet: Immunohistochemical staining was carried out for COL-I (Proteintech, 28083-1-AP, USA).

Techniques: In Vivo, Micro-CT, Staining, Immunohistochemical staining, Immunofluorescence